Characterization of Osteopontin gene of Bubalus bubalis

Characterization of Osteopontin gene of Bubalus bubalis.

Osteopontin, a glycoprotein, is expressed in several tissues including the mammary gland. The gene has been reported to be associated with milk and its constituents in various livestock species. This gene was sequenced in buffalo and it coded for the protein of 280 amino acids with the conserved GRGDS domain. The sequence was confirmed from the cDNA sequence derived from the mammary gland of bu...

Biochemical characterization of buffalo (Bubalus bubalis) milk lysozyme.

Lysozyme, a low-molecular weight basic protein, is an important component of the antibacterial system in milk. Lysozyme activity is higher in buffalo milk (60±3.9r 10 units/ml) than in bovine milk (29.1±1.5r10 units/ ml). Buffalo colostrum contains five-times more lysozyme activity than mature milk (Priyadarshini & Kansal, 2002a). Lysozyme activity in buffalo milk is not influenced by the parit...

Isolation and characterization of luteal cells in buffalo (Bubalus bubalis).

The objective of the study was to evaluate the morphological and functional characteristics of luteal cells isolated from buffalo ovary. Luteal cells exhibited columnar morphology and contact inhibition at the stage of confluence. Protein concentrations increased linearly from Day 3 to Day 7 of culture. DNA concentrations increased from Day 3 to Day 5 and then declined to Day 7 of culture. PGF2...

Isolation and characterization of buffalo (bubalus bubalis) amniotic

Characterization and In Silico Analysis of Pregnancy-Associated Glycoprotein-1 Gene of Buffalo (Bubalus bubalis)

Pregnancy-Associated Glycoproteins (PAGs) are trophoblastic proteins belonging to the Aspartic proteinase family secreted by different placental cells of many mammalian species. They play a pivotal role in placentogenesis, foetomaternal unit remodeling, and implantation. The identification of the genes encoding those proteins will be helpful to unravel the intricate embryogenomic functions duri...